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KMID : 1094720060110030179
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Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 3 p.179 ~ p.186
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Recent Advances in DNA Sequencing by End-Labeled Free-Solution Electrophoresis (ELFSE)
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Won Jong-In
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Abstract
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End-Labeled Free-Solution Electrophoresis (ELFSE) is a new technique that is a promising bioconjugate method for DNA sequencing (or separation) and genotyping by both capillary and microfluidic device electrophoresis. Because ELFSE enables high-resolution electrophoretic separation in aqueous buffer alone (i.e., without a polymer matrix), it eliminates the need to load viscous polymer networks into electrophoresis microchannels. To achieve microchannel DNA separations with high performance, ELFSE requires monodisperse perturbing entities (i.e., drag-tags), which create a large amount of frictional drag when pulled behind DNA during free-solution electrophoresis, and which have other properties suitable for microchannel electrophoresis. In this article, the theoretical concepts of ELFSE and the required characteristics of the drag-tag molecules for the ultimate performance of ELFSE are reviewed. Additionally, the merits and limitations of current drag-tags are also discussed in the context of recent experimental data of ELFSE separation (or sequencing).
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KEYWORD
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DNA sequencing, capillary electrophoresis (CE), microfluidic device, ELFSE, drag-tag, microchannel electrophoresis
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